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rabbit anti runx2 polyclonal ab  (Proteintech)


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    Structured Review

    Proteintech rabbit anti runx2 polyclonal ab
    (A) Western blots revealing <t>Runx2</t> and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.
    Rabbit Anti Runx2 Polyclonal Ab, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+wnt3a+polyclonal+ab/Animal-free+Recombinant+Human+Wnt3A/pmc11911064-132-42-48
    Average 95 stars, based on 102 article reviews
    rabbit anti runx2 polyclonal ab - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament"

    Article Title: Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament

    Journal: JBMR Plus

    doi: 10.1093/jbmrpl/ziaf021

    (A) Western blots revealing Runx2 and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.
    Figure Legend Snippet: (A) Western blots revealing Runx2 and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.

    Techniques Used: Western Blot, Expressing, Derivative Assay, Control, Cell Culture

    Related Articles

    Incubation:

    Article Title: Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament
    Article Snippet: The total protein (10 μg per well) was separated on 12.5% SDS-PAGE and transferred onto polyvinylidene difluoride membrane (PE Applied Biosystems) for 70 min. .. The membranes were washed twice in PBS solution containing 0.05% Tween 20, then blocked by a mixture of 5% skimmed milk in PBS for 1 h at room temperature, and finally incubated overnight with one of the following antibodies at 4 °C: rabbit anti-Runx2 polyclonal Ab (dilution, 1:250, Proteintech) and rabbit anti-Wnt3a polyclonal Ab (dilution, 1:250, Proteintech) to assess the osteogenic differentiation, and rabbit anti-COLIVA1 polyclonal Ab (dilution, 1:250, GeneTex) rabbit anti-FMNL3 polyclonal Ab (dilution, 1:250, Proteintech), rabbit anti-mTOR polyclonal Ab (dilution, 1:250, Proteintech), or rabbit anti-PIP4K2B polyclonal Ab (dilution, 1:250, Proteintech) to confirm the differential expression of candidate proteins. .. After washing 3 times in 0.1 M PBS, the membranes were incubated for 1 hr in anti-rabbit secondary IgG/ HRP complex (dilution, 1:5000) (Santa Cruz Biotechnology).

    Quantitative Proteomics:

    Article Title: Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament
    Article Snippet: The total protein (10 μg per well) was separated on 12.5% SDS-PAGE and transferred onto polyvinylidene difluoride membrane (PE Applied Biosystems) for 70 min. .. The membranes were washed twice in PBS solution containing 0.05% Tween 20, then blocked by a mixture of 5% skimmed milk in PBS for 1 h at room temperature, and finally incubated overnight with one of the following antibodies at 4 °C: rabbit anti-Runx2 polyclonal Ab (dilution, 1:250, Proteintech) and rabbit anti-Wnt3a polyclonal Ab (dilution, 1:250, Proteintech) to assess the osteogenic differentiation, and rabbit anti-COLIVA1 polyclonal Ab (dilution, 1:250, GeneTex) rabbit anti-FMNL3 polyclonal Ab (dilution, 1:250, Proteintech), rabbit anti-mTOR polyclonal Ab (dilution, 1:250, Proteintech), or rabbit anti-PIP4K2B polyclonal Ab (dilution, 1:250, Proteintech) to confirm the differential expression of candidate proteins. .. After washing 3 times in 0.1 M PBS, the membranes were incubated for 1 hr in anti-rabbit secondary IgG/ HRP complex (dilution, 1:5000) (Santa Cruz Biotechnology).



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    rabbit anti runx2 polyclonal ab  (Proteintech)
    95
    Proteintech rabbit anti runx2 polyclonal ab
    (A) Western blots revealing <t>Runx2</t> and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.
    Rabbit Anti Runx2 Polyclonal Ab, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+wnt3a+polyclonal+ab/Animal-free+Recombinant+Human+Wnt3A/pmc11911064-132-42-48
    Average 95 stars, based on 1 article reviews
    rabbit anti runx2 polyclonal ab - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    rabbit anti wnt3a polyclonal ab  (Proteintech)
    95
    Proteintech rabbit anti wnt3a polyclonal ab
    (A) Western blots revealing Runx2 and <t>Wnt3a</t> expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.
    Rabbit Anti Wnt3a Polyclonal Ab, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+wnt3a+polyclonal+ab/Animal-free+Recombinant+Human+Wnt3A/pmc11911064-132-50-56
    Average 95 stars, based on 1 article reviews
    rabbit anti wnt3a polyclonal ab - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Western blots revealing Runx2 and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.

    Journal: JBMR Plus

    Article Title: Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament

    doi: 10.1093/jbmrpl/ziaf021

    Figure Lengend Snippet: (A) Western blots revealing Runx2 and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.

    Article Snippet: The membranes were washed twice in PBS solution containing 0.05% Tween 20, then blocked by a mixture of 5% skimmed milk in PBS for 1 h at room temperature, and finally incubated overnight with one of the following antibodies at 4 °C: rabbit anti-Runx2 polyclonal Ab (dilution, 1:250, Proteintech) and rabbit anti-Wnt3a polyclonal Ab (dilution, 1:250, Proteintech) to assess the osteogenic differentiation, and rabbit anti-COLIVA1 polyclonal Ab (dilution, 1:250, GeneTex) rabbit anti-FMNL3 polyclonal Ab (dilution, 1:250, Proteintech), rabbit anti-mTOR polyclonal Ab (dilution, 1:250, Proteintech), or rabbit anti-PIP4K2B polyclonal Ab (dilution, 1:250, Proteintech) to confirm the differential expression of candidate proteins.

    Techniques: Western Blot, Expressing, Derivative Assay, Control, Cell Culture

    (A) Western blots revealing Runx2 and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.

    Journal: JBMR Plus

    Article Title: Proteomic analysis and effects on osteogenic differentiation of exosomes from patients with ossification of the spinal ligament

    doi: 10.1093/jbmrpl/ziaf021

    Figure Lengend Snippet: (A) Western blots revealing Runx2 and Wnt3a expression at wk 2 and 3 following culture in osteogenic differentiation medium using exosome-penetrating (1.2 μm) and exosome-non-penetrating (0.03 μm) filters. (B) Expression levels of Runx2 and Wnt3a of OLF-derived and non-OLF-derived (control) cells co-cultured with 0.03- and 1.2-μm filters. ( n = 3 each). ** p <.01.

    Article Snippet: The membranes were washed twice in PBS solution containing 0.05% Tween 20, then blocked by a mixture of 5% skimmed milk in PBS for 1 h at room temperature, and finally incubated overnight with one of the following antibodies at 4 °C: rabbit anti-Runx2 polyclonal Ab (dilution, 1:250, Proteintech) and rabbit anti-Wnt3a polyclonal Ab (dilution, 1:250, Proteintech) to assess the osteogenic differentiation, and rabbit anti-COLIVA1 polyclonal Ab (dilution, 1:250, GeneTex) rabbit anti-FMNL3 polyclonal Ab (dilution, 1:250, Proteintech), rabbit anti-mTOR polyclonal Ab (dilution, 1:250, Proteintech), or rabbit anti-PIP4K2B polyclonal Ab (dilution, 1:250, Proteintech) to confirm the differential expression of candidate proteins.

    Techniques: Western Blot, Expressing, Derivative Assay, Control, Cell Culture